Development and use of species-specific oligonucleotide probes for differentiation of Streptococcus uberis and Streptococcus parauberis

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Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.

Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis an...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1993

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.31.1.57-60.1993